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Transboundary particulate matter, photo-oxidants, acidifying and eutrophying components
Norwegian Meteorological Institute
2019
ICOS Norway – a carbon cycle infrastructure
The Integrated Carbon Observation System (ICOS) research infrastructure is aimed at quantifying and understanding the greenhouse gas balance of Europe and neighboring regions. ICOS-Norway brings together the leading
Norwegian institutes for greenhouse gas observations in the three Earth system domains atmosphere, ocean, and
terrestrial ecosystems, providing world-leading competence, which is integrated into one jointly funded and operated infrastructure. This provides Norway with a state-of-the-art research infrastructure embedded in European
and global efforts. Even though each Earth system domain was part of dedicated research infrastructures prior to
the establishment of ICOS-Norway, the greenhouse gas community in Norway was divided and there was minimal
collaboration across the Earth system domains. The overall goal of ICOS-Norway is to provide accurate and accessible data on, as well as integrated assessments of, the Norwegian carbon balance at regional scale, across the land,
ocean, and atmosphere. ICOS-Norway has thus led to an increased impact of environmental observing systems
in Norway and surrounding seas, easily seen through the number of publications and new proposals generated
as collaborative efforts. This poster presents the ICOS-Norway infrastructure, including plans for expansion and
long-term funding.
2019
The comet assay applied to HepG2 liver spheroids
In accordance with the 3 Rs to reduce in vivo testing, more advanced in vitro models, moving from 2D monolayer to 3D cultures, should be developed for prediction of human toxicity of industrial chemicals and environmental pollutants. In this study we compared cytotoxic and genotoxic responses induced by chemicals in 2D and 3D spheroidal cultures of the human liver cancer cell line HepG2.
HepG2 spheroids were prepared by hanging drop technology. Both 3D spheroids and 2D monolayer cultures were exposed to different chemicals (colchicine, chlorpromazine hydrochloride or methyl methanesulfonate) for geno- and cytotoxicity studies. Cytotoxicity was investigated by alamarBlue assay, flow cytometry and confocal imaging. DNA damage was investigated by the comet assay with and without Fpg enzyme for detection of DNA strand breaks and oxidized or alkylated base lesions.
The results from the cyto- and genotoxicity tests showed differences in sensitivity comparing the 2D and 3D HepG2 models. This study shows that human 3D spheroidal hepatocellular cultures can be successfully applied for genotoxicity testing by the comet assay and represent a promising advanced in vitro model for toxicity testing.
2019
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