Fant 9759 publikasjoner. Viser side 199 av 391:
Lack of mutagenicity of TiO2 nanoparticles in vitro despite cellular and nuclear uptake
The potential genotoxicity of titanium dioxide (TiO2) nanoparticles (NPs) is a conflictive topic because both positive and negative findings have been reported. To add clarity, we have carried out a study with two cell lines (V79–4 and A549) to evaluate the effects of TiO2 NPs (NM-101), with a diameter ranging from 15 to 60 nm, at concentrations 1–75 μg/cm2. Using two different dispersion procedures, cell uptake was determined by Transmission Electron Microscopy (TEM). Mutagenicity was evaluated using the Hprt gene mutation test, while genotoxicity was determined with the comet assay, detecting both DNA breaks and oxidized DNA bases (with formamidopyrimidine glycosylase - Fpg). Cell internalization, as determined by TEM, shows TiO2 NM-101 in cytoplasmic vesicles, as well as close to and inside the nucleus. Such internalization did not depend on the state of agglomeration, nor the dispersion used. In spite of such internalization, no cytotoxicity was detected in V79–4 cells (relative growth activity and plating efficiency assays) or in A549 cells (AlamarBlue assay) after exposure lasting for 24 h. However, a significant decrease in the relative growth activity was detected at longer exposure times (48 and 72 h) and at the highest concentration 75 µg/cm2. When the modified enzyme-linked alkaline comet assay was performed on A549 cells, although no significant induction of DNA damage was detected, a positive concentration-effects relationship was observed (Spearman’s correlation = 0.9, p 0.0001). Furthermore, no significant increase of DNA oxidized purine bases was observed. When the frequency of Hprt gene mutants was determined in V79–4 cells, no increase was observed in the exposed cells, relative to the unexposed cultures. Our general conclusion is that, under our experimental conditions, TiO2 NM-101 exposure does not exert mutagenic effects despite the evidence of NP uptake by V79–4 cells.
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In the project there were monitoring of mercury (Hg) in air at one station and sampling and analysis of Hg in precipitation at two stations as well as analysis of Hg in 14 fish from the Pasvik water course. The monitoring results for Hg in air in Karpdalen show background levels around 1,3 - 1,4 ng/m3. Enhanced concentrations of Hg correspond to enhanced concentrations of SO2 (episodes). It is therefore likely that there exist a small local source of Hg in the border areas co-located with the sources of SO2. Concentrations and deposition of Hg in precipitation are higher at Svanvik than in Karpdalen, but lower than background stations in Southern Norway. Analysis of Hg in fish meat show that the largest fish (pike, trout, perch) have concentrations higher than the threshold value of 0,5 mg/kg. White fish showed low values.
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Målet er å belyse hvilke kvalitetskrav som bør stilles til luftkvalitetsberegninger, kart og data til ulike bruksformål slik disse er formulert i norsk regelverk, herunder Forurensningsloven, Folkehelseloven, Plan- og bygningsloven, Retningslinje for behandling av luftkvalitet i arealplanlegging (T1520) m.fl. Miljødirektoratet ønsker å sikre god, enhetlig og sammenlignbar kvalitet og praksis på utredninger av luftkvalitet og har bedt om anbefalinger i form av denne rapporten.
For å få et best mulig grunnlag for å vurdere behovet for veiledning og kvalitetskriterier for ulike anvendelser, har det vært fokus på å innhente informasjon og innspill fra ulike brukergrupper og fagmiljøer. Det er gjennomført en spørreundersøkelse og det er arrangert flere møter og åpne kommentarrunder med ulike brukergrupper og fagmiljøer. Siden eventuelle kvalitetskrav vil kunne ha betydning for konkurranse i markedet og påvirke ressursbruken både hos private aktører og myndigheter, har brukermedvirkning vært et viktig element i dette arbeidet etter ønske fra oppdragsgiver.
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