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Preclinical validation of human recombinant glutamate-oxaloacetate transaminase for the treatment of acute ischemic stroke

Pérez-Mato, María; Dopico-López, Antonio; Akkoc, Yunus; López-Amoedo, Sonia; Correa-Paz, Clara; Candamo-Lourido, María; Iglesias-Rey, Ramón; López-Arias, Esteban; Bugallo-Casal, Ana; Silva-Candal, Andrés da; Bravo, Susana B.; Chantada-Vázquez, María del Pilar; Arias, Susana; Santamaría-Cadavid, María; Estany-Gestal, Ana; Zaghmi, Ahlem; Gauthier, Marc A.; Gutiérrez-Fernández, María; Martin, Abraham; Llop, Jordi; Rodríguez, Cristina; Almeida, Ángeles; Migliavacca, Martina; Polo, Ester; Pelaz, Beatriz; Gozuacik, Devrim; Yamani, Naouale El; Sengupta, Tanima; Rundén-Pran, Elise; Vivancos, José; Castellanos, Mar; Díez-Tejedor, Exuperio; Sobrino, Tomás; Rabinkov, Aharon; Mirelman, David; Castillo, José; Campos, Francisco

The blood enzyme glutamate-oxaloacetate transaminase (GOT) has been postulated as an effective therapeutic to protect the brain during stroke. To demonstrate its potential clinical utility, a new human recombinant form of GOT (rGOT) was produced for medical use.

We tested the pharmacokinetics and evaluated the protective efficacy of rGOT in rodent and non-human primate models that reflected clinical stroke conditions.

We found that continuous intravenous administration of rGOT within the first 8 h after ischemic onset significantly reduced the infarct size in both severe (30%) and mild lesions (48%). Cerebrospinal fluid and proteomics analysis, in combination with positron emission tomography imaging, indicated that rGOT can reach the brain and induce cytoprotective autophagy and induce local protection by alleviating neuronal apoptosis.

Our results suggest that rGOT can be safely used immediately in patients suspected of having a stroke. This study requires further validation in clinical stroke populations.

2024

Predicting and validating the tracking of a volcanic ash cloud during the 2006 eruption of Mt. Augustine Volcano.

Webley, P.W.; Atkinson, D.; Collins, R.L.; Dean, K.; Fochesatto, J.; Sassen, K.; Cahill, C.F.; Prata, A.; Flynn, C.J.; Mizutani, K.

2008

Predicting Future Condition and Conservation Costs from Modelling Improvements to the Indoor Environment: The Monumental Munch-Paintings in the University of Oslo’s Aula Assembly Hall

Grøntoft, Terje; Stoveland, Lena Porsmo; Frøysaker, Tine

The aim of this work was to assess how improvements to the indoor environment could affect the future condition, frequency and costs of major conservation-cleaning campaigns on the monumental paintings (1909–1916) by Edvard Munch, centrally located in the Aula assembly hall of the University of Oslo. A lower soiling rate is expected to reduce the need for frequent and major cleaning campaigns. Estimations were performed using the freely available NILU-EnvCul web-model. The conservation of these large, mostly unvarnished, oil paintings is challenging, and it is important to understand the potential benefits of preventive conservation measures. The results from the model suggested benefits from preventive conservation in protecting the paintings, and as a cost-efficient strategy to reduce the soiling and cleaning frequency. The model results indicated that an improvement in the indoor air quality in the Aula, of 50–80% as compared to the 1916–2009 average, would increase the time until the next similar major conservation cleaning campaign from approximately 45 years to between about 85 and 165 years. This should give a 45–70% reduction in the respective conservation costs. This saving was probably initiated by improvements in the recent past, before the last Aula campaign in 2009–11.

2019

Predicting the student's perceptions of multi-domain environmental factors in a Norwegian school building: Machine learning approach

Alam, Azimil Gani; Bartonova, Alena; Høiskar, Britt Ann Kåstad; Fredriksen, Mirjam; Sharma, Jivitesh; Mathisen, Hans Martin; Yang, Zhirong; Gustavsen, Kai; Hart, Kent; Fredriksen, Tore; Cao, Guangyu

Poor Indoor Environmental Quality (IEQ) in schools significantly impacts students’ well-being, learning capabilities, and health. Perceived dissatisfaction rates (PD%) among students often remain high, even when indoor environmental variables appear well-controlled. This study aims to predict perceived dissatisfaction rates (PD%) across multi-domain environmental factors—thermal, acoustic, visual, and indoor air quality (IAQ)—using machine learning (ML) models. The research integrates sensor-based environmental measurements, outdoor weather data, building parameters, and 1437 student survey responses collected from three classrooms in a Norwegian school across multiple seasons. Statistical tests were used to pre-select relevant input variables, followed by the development and evaluation of multiple ML algorithms. Among the tested ML models, Random Forest (RF) demonstrated the highest predictive accuracy for PD%, outperforming multi-linear regression (MLR) and decision trees (DT), with R² values up to 0.91 for overall IEQ dissatisfaction (PDIEQ%). SHAP analysis revealed key predictors: CO₂ levels, VOCs, humidity, temperature, solar radiation, and room window orientation. IAQ, thermal comfort, and acoustic environment were the most influential factors affecting students' perceived well-being. Despite limitations as implementation in building level scale, the study demonstrates the feasibility of deploying predictive ML models under real-world constraints for improving IEQ monitoring system. The findings support practical strategies for adaptive indoor environmental management, particularly in educational settings, and provide a replicable framework for future research. Future research can expand to other climates, buildings, measurements, occupant levels, and ML training optimization.

2025

Predictors of per- and polyfluoroalkyl substances in a maternal population from Northern Norway.

Berg, V.; Nøst, T.H.; Huber, S.; Rylander, C.; Hansen, S.; Odland, J.Ø.; Sandanger, T.M.

2014

Preface: Supplement on ELOISE II.

Pirrone, N.; Pacyna, J.M.; Munthe, J.; Barth, H.

2003

Preliminary assessment report on the spatial mapping of air quality trends for Europe. ETC/ACC Tecnical paper, 2008/3

Denby, B.; Sundvor, I.; de Smet, P.; de Leeuw, F.

2008

Preliminary assessments under the 4th daughter directive. ETC/ACC Technical paper 2007/10

Barrett, K.

2007

Preliminary evaluation of the relationship between IAQ and demand controlled ventilation.

Lopez-Aparicio, S.; Vogt, M.; Hak, C.; Dauge, F. R.; Holøs, S.; Mysen, M.

2017

Preliminary results from the evaluation of the impact of bioethanol buses on urban air quality. NILU PP

Lopez-Aparicio, S.; Hak, C.; Schmidbauer, N.; Dye, C.; Manø, S.

2012

Preliminary results of the ACTRIS ACSM intercomparison study at the SIRTA French Atmospheric Supersite in the region of Paris. NILU PP

Crenn, V.; Frölich, R.; Sciare, J.; Croteau, P.L.; Favez, O.; Verlhac, S.; Belis, C.A.; Aas, W.; Äijälä, M.; Artiñano, B.; Baisnée, D.; Baltensprenger, U.; Bonnaire, N.; Bressi, M.; Canagaratna, M.; Canonaco, F.; Carbone, C.; Cavalli, F.; Coz, E.; Cubison, M.J.; Gietl, J.K.; Green, D.C.; Gros, V.; Heikkinen, L.; Lunder, C.; Minguillón, M.C.; Mocnik, G.; O'Dowd, C.D.; Ovadnevaite, J.; Petit, J-E.; Petralia, E.; Poulain, L.; Prevôt, A.S.H.; Priestman, M.; Riffault, V.; Ripoll, A.; Sarda-Estève, R.; Slowik, J.G.; Setyan, A.; Jayne, J.T.

2014

Prenatal exposure to persistent organic pollutants and child overweight/obesity at 5-year follow-up: A prospective cohort study

Lauritzen, Hilde Brun; Larose, Tricia L; Øien, Torbjørn; Sandanger, Torkjel M; Odland, Jon Øyvind; Bor, Margot van de; Jacobsen, Geir Wenberg

Background Prenatal exposure to persistent organic pollutants (POPs), may influence offspring weight gain. More prospective epidemiological studies are needed to compliment the growing body of evidence from animal studies. Methods Serum from 412 pregnant Norwegian and Swedish women participating in a Scandinavian prospective cohort study were collected in 1986–88, and analyses of two perfluoroalkyl substances (PFASs) and five organochlorines (OCs) were conducted. We used linear and logistic regression models with 95% confidence intervals (CIs) to evaluate the associations between maternal serum POP concentrations at 17–20 weeks of gestation and child overweight/obesity (body mass index (BMI) ≥ 85th percentile) at 5-year follow-up. Results were further stratified by country after testing for effect modification. We also assessed potential non-monotonic dose-response (NMDR) relationships. Results In adjusted linear models, we observed increased BMI-for-age-and-sex z-score (β = 0.18, 95% CI: 0.01–0.35), and increased triceps skinfold z-score (β = 0.15, 95% CI: 0.02–0.27) in children at 5-year follow-up per ln-unit increase in maternal serum perfluorooctane sulfonate (PFOS) concentrations. We observed increased odds for child overweight/obesity (BMI ≥ 85th percentile) for each ln-unit increase in maternal serum PFOS levels (adjusted OR: 2.04, 95% CI: 1.11–3.74), with stronger odds among Norwegian children (OR: 2.96, 95% CI: 1.42–6.15). We found similar associations between maternal serum perfluorooctanoate (PFOA) concentrations and child overweight/obesity. We found indications of NMDR relationships between PFOS and polychlorinated biphenyl (PCB) 153 and child overweight/obesity among Swedish children. Conclusion We found positive associations between maternal serum PFAS concentrations and child overweight/obesity at 5-year follow-up, particularly among Norwegian participants. We observed some evidence for NMDR relationships among Swedish participants.

2018

Preparation and certification of a reference material on PCBs in pig fat and its application in quality control in monitoring laboratories during the Belgian

Bester, K.; de Vos, P.; Le Guern, L.; Harbeck, S.; Hendrickx, F.; Kramer, G.N.; Linsinger, T.; Mertens, I.; Schimmel, H.; Sejeroe-Olsen, B.; Pauwels, J.; De Poorter, G.; Rimkus, G.G.; Schlabach, M.

2001

Presentasjon av CIENS-prosjekt Kunnskapsstatus for tverrfaglig klima- og miljøforskning

Skjellum, Solrun Figenschau; Bartonova, Alena; Ruud, Audun; Slettemark, Britta

2021

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