Publikasjoner

Genotoxicity testing of nanoparticles.

Kazimirova, A.; Barancokova, M.; Wsólová, L.; Dusinska, M.

Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo.

Dobrzynska, M.M.; Gajowik, A.; Radzikowska, J.; Lankoff, A.; Dusinska, M.; Kruszewski, M.

Genotoxicity of nanomaterials. Do we have testing strategy? NILU F

Dusinska, M.; Huk, A.; El Yamani, N.; Fjellsbø, L.M.; Rinna, A.; Rundén-Pran, E.; NanoTEST team.

Genotoxicity of nanomaterials in advanced in vitro model systems

Dusinska, Maria; Elje, Elisabeth; Mariussen, Espen; Gutleb, A.; Serchi, T.; Rundén-Pran, Elise

Genotoxicity of engineered nanoparticles.

Dusinska, M.; El Yamani, N.; Elje, E.; Rundén-Pran, E.

Genotoxicity assessment of nanomaterials in advanced lung models

Elje, Elisabeth; Mariussen, Espen; McFadden, Erin; Camassa, Laura Maria Azzurra; Dusinska, Maria; Zienolddiny, Shanbeh; Rundén-Pran, Elise

Genotoxicity Assessment Of Nanomaterials In Advanced Liver And Lung Models

Elje, Elisabeth; Mariussen, Espen; Dusinska, Maria; Rundén-Pran, Elise

Genotoxic effects of occupational exposure to glass fibres - A human biomonitoring study.

Ceppi, Marcello; Smolkova, Bozena ; Staruchova, Marta; Kazimirova, Alena; Barancokova, Magdalena; Volkovova, Katarina; Collins, Andrew Richard; Kocan, Anton; Dzupinkova, Zuzana; Horska, Alexandra; Buocikova, Verona; Tulinska, Jana; Liskova, Aurelia; Mikusova, Miroslava Lehotska; Krivosikova, Zora; Wsolova, Ladislava; Kuba, Daniel; Rundén-Pran, Elise; El Yamani, Naouale; Longhin, Eleonora Marta; Halasova, Erika; Kyrtopoulos, Soterios; Bonassi, Stefano ; Dusinska, Maria

As part of a large human biomonitoring study, we conducted occupational monitoring in a glass fibre factory in Slovakia. Shopfloor workers (n = 80), with a matched group of administrators in the same factory (n = 36), were monitored for exposure to glass fibres and to polycyclic aromatic hydrocarbons (PAHs). The impact of occupational exposure on chromosomal aberrations, DNA damage and DNA repair, immunomodulatory markers, and the role of nutritional and lifestyle factors, as well as the effect of polymorphisms in metabolic and DNA repair genes on genetic stability, were investigated.

The (enzyme-modified) comet assay was employed to measure DNA strand breaks (SBs) and apurinic sites, oxidised and alkylated bases. Antioxidant status was estimated by resistance to H2O2-induced DNA damage. Base excision repair capacity was measured with an in vitro assay (based on the comet assay).

Exposure of workers to fibres was low, but still was associated with higher levels of SBs, and SBs plus oxidised bases, and higher sensitivity to H2O2. Multivariate analysis showed that exposure increased the risk of high levels of SBs by 20%. DNA damage was influenced by antioxidant enzymes catalase and glutathione S-transferase (measured in blood). DNA repair capacity was inversely correlated with DNA damage and positively with antioxidant status. An inverse correlation was found between DNA base oxidation and the percentage of eosinophils (involved in the inflammatory response) in peripheral blood of both exposed and reference groups. Genotypes of XRCC1 variants rs3213245 and rs25487 significantly decreased the risk of high levels of base oxidation, to 0.50 (p = 0.001) and 0.59 (p = 0.001), respectively.

Increases in DNA damage owing to glass fibre exposure were significant but modest, and no increases were seen in chromosome aberrations or micronuclei. However, it is of concern that even low levels of exposure to these fibres can cause significant genetic damage.

Genetic variation in the major mitotic checkpoint genes associated with chromosomal aberrations in healthy humans.

Försti, A.; Frank, C.; Smolkova, B.; Kazimirova, A.; Barancokova, M.; Vymetalkova, V.; Kroupa, M.; Naccarati, A.; Vodickova, L.; Buchancova, J.; Dusinska, M.; Musak, L.; Vodicka, P.; Hemminki, K.

Genetic variability in biotransformation enzymes and immune response parameters in people exposed to mineral wool.

Horska, A.; Kuricova, M.; Tulinska, J.; Liskova, A.; Volkovová, K.; Wsólová, L.; Dusinska, M.

Genetic determinants of quantitative traits associated with cardiovascular disease risk.

Smolkova, B.; Bonassi, S.; Buocikova, V.; Dusinska, M.; Horska, A.; Kuba, D.; Dzupinkova, Z.; Raslova, K.; Gašparovic, J.; Slíz, I.; Ceppi, M.; Vohnout, B.; Wsolova, L.; Volkovova, K.

Generation of testable adverse outcome pathways (AOPs) for nanomaterial human hazard assessment

Murugadoss, Sivakumar; Vincovik Vrcek, Ivana; Cimpan, Mihaela-Roxana; Martens, Marvin; Gromelski, Maciej; Puzyn, Tomasz; Fessard, Valérie; Lynch, Iseult; Dusinska, Maria; Hoet, Peter