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Investigating relationships between exposure to organohalogenated contaminants and biomarkers of health in a marine top predator - exploring the toolbox

Hansen, Elisabeth; Bustnes, Jan Ove; Herzke, Dorte; Helander, Björn; Criscuolo, François; Huber, N; Eulaers, Igor; Sun, J.; Bourgeon, Sophie

2022

Monitoring of long-range transported air pollutants in Norway. Annual Report 2021.

Aas, Wenche; Berglen, Tore Flatlandsmo; Eckhardt, Sabine; Fiebig, Markus; Solberg, Sverre; Yttri, Karl Espen

This report presents results from the monitoring of atmospheric composition and deposition of air pollution in 2021, and focuses on main components in air and precipitation, particulate and gaseous phase of inorganic constituents, particulate carbonaceous matter, ground level ozone and particulate matter. The level of pollution in 2021 was generally low with few high episodes.

NILU

2022

Inferring surface energy fluxes using drone data assimilation in large eddy simulations

Pirk, Norbert; Aalstad, Kristoffer; Westermann, Sebastian; Vatne, Astrid; van Hove, Alouette; Tallaksen, Lena Merete; Cassiani, Massimo; Katul, Gabriel G.

Spatially representative estimates of surface energy exchange from field measurements are required for improving and validating Earth system models and satellite remote sensing algorithms. The scarcity of flux measurements can limit understanding of ecohydrological responses to climate warming, especially in remote regions with limited infrastructure. Direct field measurements often apply the eddy covariance method on stationary towers, but recently, drone-based measurements of temperature, humidity, and wind speed have been suggested as a viable alternative to quantify the turbulent fluxes of sensible (H) and latent heat (LE). A data assimilation framework to infer uncertainty-aware surface flux estimates from sparse and noisy drone-based observations is developed and tested using a turbulence-resolving large eddy simulation (LES) as a forward model to connect surface fluxes to drone observations. The proposed framework explicitly represents the sequential collection of drone data, accounts for sensor noise, includes uncertainty in boundary and initial conditions, and jointly estimates the posterior distribution of a multivariate parameter space. Assuming typical flight times and observational errors of light-weight, multi-rotor drone systems, we first evaluate the information gain and performance of different ensemble-based data assimilation schemes in experiments with synthetically generated observations. It is shown that an iterative ensemble smoother outperforms both the non-iterative ensemble smoother and the particle batch smoother in the given problem, yielding well-calibrated posterior uncertainty with continuous ranked probability scores of 12 W m−2 for both H and LE, with standard deviations of 37 W m−2 (H) and 46 W m−2 (LE) for a 12 min vertical step profile by a single drone. Increasing flight times, using observations from multiple drones, and further narrowing the prior distributions of the initial conditions are viable for reducing the posterior spread. Sampling strategies prioritizing space–time exploration without temporal averaging, instead of hovering at fixed locations while averaging, enhance the non-linearities in the forward model and can lead to biased flux results with ensemble-based assimilation schemes. In a set of 18 real-world field experiments at two wetland sites in Norway, drone data assimilation estimates agree with independent eddy covariance estimates, with root mean square error values of 37 W m−2 (H), 52 W m−2 (LE), and 58 W m−2 (H+LE) and correlation coefficients of 0.90 (H), 0.40 (LE), and 0.83 (H+LE). While this comparison uses the simplifying assumptions of flux homogeneity, stationarity, and flat terrain, it is emphasized that the drone data assimilation framework is not confined to these assumptions and can thus readily be extended to more complex cases and other scalar fluxes, such as for trace gases in future studies.

2022

Comparing national greenhouse gas budgets reported in UNFCCC inventories against atmospheric inversions

Deng, Zhu; Ciais, Philippe; Tzompa-Sosa, Zitely A.; Saunois, Marielle; Qiu, Chunjing; Tan, Chang; Sun, Taochun; Ke, Piyu; Cui, Yanan; Tanaka, Katsumasa; Lin, Xin; Thompson, Rona Louise; Tian, Hanqin; Yao, Yuanzhi; Huang, Yuanyuan; Lauerwald, Ronny; Jain, Atul K.; Xu, Xiaoming; Bastos, Ana; Palmer, Paul I.; Lauvaux, Thomas; d'Aspremont, Alexandre; Giron, Clément; Benoit, Antoine; Poulter, Benjamin; Chang, Jinfeng; Petrescu, Ana Maria Roxana; Davis, Steven J; Liu, Zhu; Grassi, Giacomo; Albergel, Clement; Tubiello, Francesco N. ; Perugini, Lucia; Peters, Wouter; Chevallier, Frederic

In support of the global stocktake of the Paris Agreement on climate change, this study presents a comprehensive framework to process the results of an ensemble of atmospheric inversions in order to make their net ecosystem exchange (NEE) carbon dioxide (CO2) flux suitable for evaluating national greenhouse gas inventories (NGHGIs) submitted by countries to the United Nations Framework Convention on Climate Change (UNFCCC). From inversions we also deduced anthropogenic methane (CH4) emissions regrouped into fossil and agriculture and waste emissions, as well as anthropogenic nitrous oxide (N2O) emissions. To compare inversion results with national reports, we compiled a new global harmonized database of emissions and removals from periodical UNFCCC inventories by Annex I countries, and from sporadic and less detailed emissions reports by non-Annex I countries, given by national communications and biennial update reports. No gap filling was applied. The method to reconcile inversions with inventories is applied to selected large countries covering ∼90 % of the global land carbon uptake for CO2 and top emitters of CH4 and N2O. Our method uses results from an ensemble of global inversions produced by the Global Carbon Project for the three greenhouse gases, with ancillary data. We examine the role of CO2 fluxes caused by lateral transfer processes from rivers and from trade in crop and wood products and the role of carbon uptake in unmanaged lands, both not accounted for by NGHGIs. Here we show that, despite a large spread across the inversions, the median of available inversion models points to a larger terrestrial carbon sink than inventories over temperate countries or groups of countries of the Northern Hemisphere like Russia, Canada and the European Union. For CH4, we find good consistency between the inversions assimilating only data from the global in situ network and those using satellite CH4 retrievals and a tendency for inversions to diagnose higher CH4 emission estimates than reported by NGHGIs. In particular, oil- and gas-extracting countries in central Asia and the Persian Gulf region tend to systematically report lower emissions compared to those estimated by inversions. For N2O, inversions tend to produce higher anthropogenic emissions than inventories for tropical countries, even when attempting to consider only managed land emissions. In the inventories of many non-Annex I countries, this can be tentatively attributed to a lack of reporting indirect N2O emissions from atmospheric deposition and from leaching to rivers, to the existence of natural sources intertwined with managed lands, or to an underestimation of N2O emission factors for direct agricultural soil emissions. Inversions provide insights into seasonal and interannual greenhouse gas fluxes anomalies, e.g., during extreme events such as drought or abnormal fire episodes, whereas inventory methods are established to estimate trends and multi-annual changes. As a much denser sampling of atmospheric CO2 and CH4 concentrations by different satellites coordinated into a global constellation is expected in the coming years, the methodology proposed here to compare inversion results with inventory reports (e.g., NGHGIs) could be applied regularly for monitoring the effectiveness of mitigation policy and progress by countries to meet the objective of their pledges. The dataset constructed by this study is publicly available at https://doi.org/10.5281/zenodo.5089799 (Deng et al., 2021).

2022

Hazard Assessment of Benchmark Metal-Based Nanomaterials Through a Set of In Vitro Genotoxicity Assays

Vital, Nádia; Pinhão, Mariana; El Yamani, Naouale; Rundén-Pran, Elise; Louro, Henriqueta; Dusinska, Maria; Silva, Maria João

2022

Målinger av miljøgifter i luft ved Franzefoss Eide på Sotra og Husøya ved Kristiansund

Berglen, Tore Flatlandsmo; Vik, Aasmund Fahre; Mortensen, Tore; Andresen, Erik; Bæk, Kine; Davanger, Kirsten; Enge, Ellen Katrin; Gundersen, Hans; Hanssen, Linda; Harju, Mikael; Nipen, Maja; Bohlin-Nizzetto, Pernilla; Röhler, Laura; Schmidbauer, Norbert; Skaar, Jøran Solnes

NILU har gjennomført måleprogram for konsentrasjoner i luft ved Franzefoss Gjenvinning AS sine anlegg ved Eide på Sotra og ved Husøya ved Kristiansund. Ved Eide ble det tatt prøver i luft og analysert for prioriterte miljøgifter som dekloraner, fenoler, ftalater, PFAS, benzotriazoler, organiske tinnforbindelser, samt VOC inkludert D6, ammoniakk (NH3), gassfase HCl og hydrogensulfid (H2S). For de prioriterte miljøgiftene var de fleste prøvene under deteksjonsgrensen. De høyeste verdiene ble observert ved Lokasjon 11 Vannrenseanlegget. Ved Husøya ble det tatt prøver i luft og analysert for VOC inkludert D6, ammoniakk (NH3) og gassfase HCl. Verdiene ved Husøya var lavere enn ved Eide.

NILU

2022

Spatial Variability of POPs in Air at European Background Sites

Halvorsen, Helene Lunder; Bohlin-Nizzetto, Pernilla; Breivik, Knut; Eckhardt, Sabine; Moeckel, Claudia; Gusev, Alexey; Shatalov, Victor; Skogeng, Lovise Pedersen

2022

Nye beregninger viser dobbelt så mye metan over Skandinavia

Platt, Stephen Matthew; Samset, Bjørn Hallvard (intervjuobjekter); Elster, Kristian (journalist)

2022

An NO2 sensor based on WO3 thin films for automotive applications in the microwave frequency range

Paleczek, Anna; Grochala, D.; Staszek, K.; Gruszczynski, S.; Maciak, Erwin; Opilski, Zbigniew; Kaluzynski, Piotr; Wojcikowski, Marek; Cao, Tuan-Vu; Rydosz, A.

2022

A Systematic Review of plastic analytical protocols and Reproducible Analytical Pipelines

Aliani, Stefano; Suaria, Giuseppe; Herzke, Dorte; De Witte, Bavo P.; Primpke, Sebastian; Nikiforov, Vladimir; Strand, Jakob; Da Silva, Vitor; Lusher, Amy Lorraine

2022

Metanlekkasjen fra Nord Stream

Platt, Stephen Matthew (intervjuobjekt)

2022

Initial studies to update the European AQ city viewer

Soares, Joana; Schneider, Philipp; Horálek, Jan; Malherbe, Laure; González Ortiz, Alberto; Liberti, Luca; Gsella, Artur

2022

Mapping global flying aircraft activities using Landsat 8 and cloud computing

Zhao, Fen; Xia, Lang; Kylling, Arve; Shang, Hua; Yang, Peng

Elsevier

2022

Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration

Eilertsen, Hans Christian; Eriksen, Gunilla; Bergum, John-Steinar; Strømholt, Jo; Elvevoll, Edel O.; Eilertsen, Karl-Erik; Heimstad, Eldbjørg Sofie; Giæver, Ingeborg Hulda; Israelsen, Linn; Svenning, Jon Brage; Dalheim, Lars; Osvik, Renate Døving; Hansen, Espen Holst; Ingebrigtsen, Richard Andre; Aspen, Terje M; Wintervoll, Geir-Henning

From 2015 to 2021, we optimized mass cultivation of diatoms in our own developed vertical column airlift photobioreactors using natural and artificial light (LEDs). The project took place at the ferrosilicon producer Finnfjord AS in North Norway as a joint venture with UiT—The Arctic University of Norway. Small (0.1–6–14 m3) reactors were used for initial experiments and to produce inoculum cultures while upscaling experiments took place in a 300 m3 reactor. We here argue that species cultivated in reactors should be large since biovolume specific self-shadowing of light can be lower for large vs. small cells. The highest production, 1.28 cm3 L−1 biovolume (0.09–0.31 g DW day−1), was obtained with continuous culture at ca. 19% light utilization efficiency and 34% CO2 uptake. We cultivated 4–6 months without microbial contamination or biofouling, and this we argue was due to a natural antifouling (anti-biofilm) agent in the algae. In terms of protein quality all essential amino acids were present, and the composition and digestibility of the fatty acids were as required for feed ingredients. Lipid content was ca. 20% of ash-free DW with high EPA levels, and omega-3 and amino acid content increased when factory fume was added. The content of heavy metals in algae cultivated with fume was well within the accepted safety limits. Organic pollutants (e.g., dioxins and PCBs) were below the limits required by the European Union food safety regulations, and bioprospecting revealed several promising findings.

MDPI

2022

Odds and ends of atmospheric mercury in Europe and over the North Atlantic Ocean: temporal trends of 25 years of measurements

Custódio, Danilo; Pfaffhuber, Katrine Aspmo; Spain, T. Gerard; Pankratov, Fidel F.; Strigunova, Iana; Molepo, Koketso; Skov, Henrik; Bieser, Johannes; Ebinghaus, Ralf

The global monitoring plan of the Minamata Convention on Mercury was established to generate long-term data necessary for evaluating the effectiveness of regulatory measures at a global scale. After 25 years of monitoring (since 1995), Mace Head is one of the atmospheric monitoring stations with the longest mercury record and has produced sufficient data for the analysis of temporal trends of total gaseous mercury (TGM) in Europe and the North Atlantic. Using concentration-weighted trajectories for atmospheric mercury measured at Mace Head as well as another five locations in Europe, Amderma, Andøya, Villum, Waldhof and Zeppelin, we identify the regional probabilistic source contribution factor and its changes for the period of 1996 to 2019. Temporal trends indicate that concentrations of mercury in the atmosphere in Europe and the North Atlantic have declined significantly over the past 25 years at a non-monotonic rate averaging 0.03  . Concentrations of TGM at remote marine sites were shown to be affected by continental long-range transport, and evaluation of reanalysis back trajectories displays a significant decrease in TGM in continental air masses from Europe in the last 2 decades. In addition, using the relationship between mercury and other atmospheric trace gases that could serve as a source signature, we perform factorization regression analysis, based on positive rotatable factorization to solve probabilistic mass functions. We reconstructed atmospheric mercury concentration and assessed the contribution of the major natural and anthropogenic sources. The results reveal that the observed downward trend in the atmospheric mercury is mainly associated with a factor with a high load of long-lived anthropogenic species.

2022

Understand and mitigate impacts of 3D clouds on UV-VIS NO2 trace gas retrievals by AI exploration of synthetic and real data

Kylling, Arve; Emde, Claudia; Yu, Huan; van Roozendael, Michel; Stebel, Kerstin; Mayer, Bernhard

2022

Thymidine Kinase+/− Mammalian Cell Mutagenicity Assays for Assessment of Nanomaterials

Chen, Tao; Dusinska, Maria; Elespuru, Rosalie K.

The methods outlined here are part of a series of papers designed specifically for genotoxicity assessment of nanomaterials (NM). Common Considerations such as NM characterization, sample preparation and dose selection, relevant to all genotoxicity assays, are found in an accompanying paper. The present paper describes methods for evaluation of mutagenicity in the mammalian (mouse) thymidine kinase (Tk) gene occurring in L5178Y mouse lymphoma (ML) cells and in the designated TK gene in human lymphoblastoid TK6 cells. Mutations change the functional genotype from TK+/− to TK−/−, detectable as cells surviving on media selective for the lack of thymidine kinase (TK) function. Unlike cells with TK enzyme function, the TK−/− cells are unable to integrate the toxic selection agent, allowing these cells to survive as rare mutant colonies. The ML assay has been shown to detect a broad spectrum of genetic damage, including both small scale (point) mutations and chromosomal alterations. This assay is a widely used mammalian cell gene mutation assay for regulatory purposes and is included in the core battery of genotoxicity tests for regulatory decision-making. The TK6 assay is an assay using a human cell line derived similarly via mutagenic manipulations and optimal selection. Details are provided on the materials required, cell culture methods, selection of test chemical concentrations, cytotoxicity, treatment time, mutation expression, cloning, and data calculation and interpretation. The methods describe the microwell plate version of the assays without metabolic activation.

Frontiers Media S.A.

2022

The impact of climate sensitive factors on the exposure to organohalogenated contaminants in an aquatic bird exploiting both marine and freshwater habitats

Bustnes, Jan Ove; Bårdsen, Bård-Jørgen; Herzke, Dorte; Bangjord, Georg; Bollinger, Eric; Bourgeon, Sophie; Schulz, Ralf; Fritsch, Clémentine; Eulaers, Igor

To assess how climate-sensitive factors may affect the exposure to organochlorines (OCs) and perfluoroalkyl substances (PFASs), we monitored concentrations in eggs of the common goldeneye (Bucephala clangula) over two decades (1999–2019) in central Norway. The goldeneye alternates between marine and freshwater habitats and is sensitive to climate variation, especially due to alterations in ice conditions which may affect feeding conditions. We assessed how biological factors such as diet (stable isotopes δ13C and δ15N), the onset of egg laying, and physical characteristics such as winter climate (North Atlantic Oscillation: NAOw) influenced exposure. We predicted compounds to show different temporal trends depending on whether they were still in production (i.e. some PFASs) or have been banned (i.e. legacy OCs and some PFASs). Therefore, we controlled for potential temporal trends in all analyses. There were declining trends for α- and γ-hexachlorocyclohexane (HCH), oxychlordane, cis-chlordane, cis-nonachlor, p,p′-dichlorodiphenyltrichloroethane (p.p′-DDT) and less persistent polychlorinated biphenyl (PCB) congeners (e.g. PCB101). In contrast, the dominant compounds, such as p,p′-dichlorodiphenyldichloroethylene (p,p′-DDE) and persistent PCB congeners, were stable, whereas hexachlorobenzene (HCB) increased over time. Most OCs were positively related to δ15N, suggesting higher exposure in birds feeding at upper trophic levels. Chlordanes and HCB were positively associated with δ13C, indicating traces of marine input for these compounds, whereas the relationships to most PCBs were negative. Among PFASs, perfluorooctanesulfonamide (PFOSA) and perfluorohexane sulfonic acid (PFHxS) declined. Most PFASs were positively associated with δ13C, whereas there were no associations with δ15N. Egg laying date was positively associated to perfluoroheptanesulfonic acid (PFHpS), perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), suggesting that some of the PFAS load originated from the wintering locations. Although NAOw had little impact on the exposure to organohalogenated contaminants, factors sensitive to climate change, especially diet, were associated with the exposure to OHCs in goldeneyes.

Elsevier

2022

Estimation of surface NO2 concentration over Europe using Sentinel-5P Observations and machine learning models

Shetty, Shobitha; Schneider, Philipp; Stebel, Kerstin; Hamer, Paul David; Kylling, Arve

2022

EUROqCHARM - Assuring Reproducible, Harmonised and Quality-Controlled Assessments of Plastic Pollution

van Bavel, Bert; Lusher, Amy; Aliani, Stefano; de Boer, Jacob; van der Veen, Ike; Galgani, Francois; Stoica, Elena; Fabres, Joan; Farre, Marinella; Nikiforov, Vladimir; De Witte, Bavo; Primpke, Sebastian; Strand, Jakob; Johansen, Jon Eigill; Kaegi, Ralf; Giorgetti, Alessandra; Del Cerro, Corrinne

2022

Integument carotenoid-based colouration reflects contamination to perfluoroalkyl substances, but not mercury, in arctic black-legged kittiwakes

Costantini, David; Blévin, Pierre; Bustnes, Jan Ove; Esteve, Valérie; Gabrielsen, Geir Wing; Herzke, Dorte; Humann-Guilleminot, Ségolène; Moe, Børge; Parenteau, Charline; Récapet, Charlotte; Bustamante, Paco; Chastel, Olivier

Anthropogenic activities are introducing multiple chemical contaminants into ecosystems that act as stressors for wildlife. Perfluoroalkyl substances (PFAS) and mercury (Hg) are two relevant contaminants that may cause detrimental effects on the fitness of many aquatic organisms. However, there is a lack of information on their impact on the expression of secondary sexual signals that animals use for mate choice. We have explored the correlations between integument carotenoid-based colourations, blood levels of carotenoids, and blood levels of seven PFAS and of total Hg (THg) in 50 adult male black-legged kittiwakes (Rissa tridactyla) from the Norwegian Arctic during the pre-laying period, while controlling for other colouration influencing variables such as testosterone and body condition. Kittiwakes with elevated blood concentrations of PFAS (PFOSlin, PFNA, PFDcA, PFUnA, or PFDoA) had less chromatic but brighter bills, and brighter gape and tongue; PFOSlin was the pollutant with the strongest association with bill colourations. Conversely, plasma testosterone was the only significant correlate of hue and chroma of both gape and tongue, and of hue of the bill. Kittiwakes with higher concentrations of any PFAS, but not of THg, tended to have significantly higher plasma concentrations of the carotenoids astaxanthin, zeaxanthin, lutein, and cryptoxanthin. Our work provides the first correlative evidence that PFAS exposure might interfere with the carotenoid metabolism and the expression of integument carotenoid-based colourations in a free-living bird species. This outcome may be a direct effect of PFAS exposure or be indirectly caused by components of diet that also correlate with elevated PFAS concentrations (e.g., proteins). It also suggests that there might be no additive effect of THg co-exposure with PFAS on the expression of colourations. These results call for further work on the possible interference of PFAS with the expression of colourations used in mate choice.

Frontiers Media S.A.

2022

Digital technologies for environmental footprints and product passports

Bouman, Evert Alwin; Abbasi, Golnoush; Ebrahimi, Babak

2022

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