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Measuring DNA modifications with the comet assay: a compendium of protocols

Collins, Andrew; Møller, Peter; Gajski, Goran; Vodenková, Soňa; Abdulwahed, Abdulhadi; Anderson, Diana; Bankoglu, Ezgi Eyluel; Bonassi, Stefano; Boutet-Robinet, Elisa; Brunborg, Gunnar; Chao, Christy; Cooke, Marcus S.; Costa, Carla; Costa, Solange; Dhawan, Alok; Lapuente, Joaquin de; Bo, Cristian Del; Dubus, Julien; Dusinska, Maria; Duthie, Susan J.; Yamani, Naouale El; Engelward, Bevin; Gaivão, Isabel; Giovannelli, Lisa; Godschalk, Roger; Guilherme, Sofia; Gutzkow, Kristine Bjerve; Habas, Khaled; Hernández, Alba; Herrero, Oscar; Isidori, Marina; Jha, Awadhesh N.; Knasmüller, Siegfried; Kooter, Ingeborg M.; Koppen, Gudrun; Kruszewski, Marcin; Ladeira, Carina; Laffon, Blanca; Larramendy, Marcelo; Hégarat, Ludovic Le; Lewies, Angelique; Lewinska, Anna; Liwszyc, Guillermo E.; Cerain, Adela López de; Manjanatha, Mugimane; Marcos, Ricard; Milic, Mirta; Andrade, Vanessa Moraes de; Moretti, Massimo; Muruzabal, Damian; Novak, Matjaž; Oliveira, Rui; Olsen, Ann-Karin Hardie; Owiti, Norah; Pacheco, Mário; Pandey, Alok K.; Pfuhler, Stefan; Pourrut, Bertrand; Reisinger, Kerstin; Rojas, Emilio; Rundén-Pran, Elise; Sanz-Serrano, Julen; Shaposhnikov, Sergey; Sipinen, Ville Erling; Smeets, Karen; Stopper, Helga; Teixeira, João Paulo; Valdiglesias, Vanessa; Valverde, Mahara; Acker, Frederique van; Schooten, Frederik-Jan van; Vasquez, Marie; Wentzel, Johannes F.; Wnuk, Maciej; Wouters, Annelies; Žegura, Bojana; Zikmund, Tomas; Langie, Sabine A. S.; Azqueta, Amaya

2023

Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure

Bjørneset, J.; Blévin, P.; Bjørnstad, P.M.; Dalmo, R.A.; Goksøyr, A.; Harju, M.; Limonta, G.; Panti, C.; Rikardsen, A.H.; Sundaram, A.Y.M.; Yadetie, F.; Routti, H.

Populations of killer whale (Orcinus orca) contain some of the most polluted animals on Earth. Yet, the knowledge on effects of chemical pollutants is limited in this species. Cell cultures and in vitro exposure experiments are pertinent tools to study effects of pollutants in free-ranging marine mammals. To investigate transcriptional responses to pollutants in killer whale cells, we collected skin biopsies of killer whales from the Northern Norwegian fjords and successfully established primary fibroblast cell cultures from the dermis of 4 out of 5 of them. Cells from the individual with the highest cell yield were exposed to three different concentrations of a mixture of persistent organic pollutants (POPs) that reflects the composition of the 10 most abundant POPs found in Norwegian killer whales (p,p’-DDE, trans-nonachlor, PCB52, 99, 101, 118, 138, 153, 180, 187). Transcriptional responses of 13 selected target genes were studied using digital droplet PCR, and whole transcriptome responses were investigated utilizing RNA sequencing. Among the target genes analysed, CYP1A1 was significantly downregulated in the cells exposed to medium (11.6 µM) and high (116 µM) concentrations of the pollutant mixture, while seven genes involved in endocrine functions showed a non-significant tendency to be upregulated at the highest exposure concentration. Bioinformatic analyses of RNA-seq data indicated that 13 and 43 genes were differentially expressed in the cells exposed to low and high concentrations of the mixture, respectively, in comparison to solvent control. Subsequent pathway and functional analyses of the differentially expressed genes indicated that the enriched pathways were mainly related to lipid metabolism, myogenesis and glucocorticoid receptor regulation. The current study results support previous correlative studies and provide cause-effect relationships, which is highly relevant for chemical and environmental management.

2023

Total ozone trends at three northern high-latitude stations

Bernet, Leonie; Svendby, Tove Marit; Hansen, Georg Heinrich; Orsolini, Yvan; Dahlback, Arne; Goutail, Florence; Pazmino, Andrea; Petkov, Boyan; Kylling, Arve

After the decrease of ozone-depleting substances (ODSs) as a consequence of the Montreal Protocol, it is still challenging to detect a recovery in the total column amount of ozone (total ozone) at northern high latitudes. To assess regional total ozone changes in the “ozone-recovery” period (2000–2020) at northern high latitudes, this study investigates trends from ground-based total ozone measurements at three stations in Norway (Oslo, Andøya, and Ny-Ålesund). For this purpose, we combine measurements from Brewer spectrophotometers, ground-based UV filter radiometers (GUVs), and a SAOZ (Système d'Analyse par Observation Zénithale) instrument. The Brewer measurements have been extended to work under cloudy conditions using the global irradiance (GI) technique, which is also presented in this study. We derive trends from the combined ground-based time series with the multiple linear regression model from the Long-term Ozone Trends and Uncertainties in the Stratosphere (LOTUS) project. We evaluate various predictors in the regression model and found that tropopause pressure and lower-stratospheric temperature contribute most to ozone variability at the three stations. We report significantly positive annual trends at Andøya (0.9±0.7 % per decade) and Ny-Ålesund (1.5±0.1 % per decade) and no significant annual trend at Oslo (0.1±0.5 % per decade) but significantly positive trends in autumn at all stations. Finally we found positive but insignificant trends of around 3 % per decade in March at all three stations, which may be an indication of Arctic springtime ozone recovery. Our results contribute to a better understanding of regional total ozone trends at northern high latitudes, which is essential to assess how Arctic ozone responds to changes in ODSs and to climate change.

2023

Migration analysis of chemical additives from indoor consumer plastic products

Bohlin-Nizzetto, Pernilla

NILU has, on behalf of the Norwegian Environment Agency, performed chemical analyses of a selection of chemical additives in indoor consumer plastic products. The goal was to identify content and migration of the chemical additives in and from the products to particles and surfaces of the products at room temperature. The plastic products included; furniture wrap, wall papers, table cloths, shower curtains, floor tiles, mattresses, pillows, carpet underlays and a bathtub mat. Targeted chemicals were organophosphorous flame retardants (OPFRs), brominated flame retardants (BFRs) including tetrabromobisphenol A (TBBPA), chlorinated paraffins (CPs) and dechloranes. MCCPs and SCCPs were found in ‰ to % levels in PVC containing products. Four of the OPFRs were found in up to ‰ levels in adhesive foils to be put on furniture or floor/walls and mattresses. None of the targeted brominated compounds nor melamine were detected in the product samples.

NILU

2023

Reproducible pipelines and readiness levels in plastic monitoring

Aliani, Stefano; Lusher, Amy L.; Galgani, Francois; Herzke, Dorte; Nikiforov, Vladimir; Primpke, Sebastian; Roscher, Lisa; Silva, Vitor Hugo da; Strand, Jakob; Suaria, Giuseppe; Vanavermaete, David; Verlé, Katrien; Witte, Bavo De; Bavel, Albert Van

2023

Aluminium buckets and 600 degrees help researchers find microplastics in air

Solbakken, Christine Forsetlund; Herzke, Dorte; Gabrielsen, Geir Wing; Hallanger, Ingeborg G.

2023

What should we do while waiting for environmental pollutants to be banned?

Solbakken, Christine Forsetlund; Heimstad, Eldbjørg Sofie; Hanssen, Linda

2023

Plastic contamination in fulmars in the European Arctic over 25 years

Collard, France; Tulatz, Felix; Husabø, Eirin; Benjaminsen, Stine Charlotte; Krapp, Rupert; Strøm, Hallvard; Gabrielsen, Geir Wing; Herzke, Dorte; Harju, Mikael; Halsband, Claudia; Sagerup, Kjetil; Tarroux, Arnaud; Danielsen, Jóhannis; Anderssen, Kathryn Elizabeth

2023

Ingestion of car tyre rubber by lumpfish increases exposure to toxins

Hägg, Fanny; Halsband, Claudia; Herzke, Dorte; Nikiforov, Vladimir; Booth, Andrew Michael; Bourgeon, Sophie

2023

Slik vinn du «støv-krigen»

Bohlin-Nizzetto, Pernilla (intervjuobjekt); Nørstebø, Randi (journalist)

2023

Revised Historical Black Carbon Emissions based on Atmospheric Modelling, Ice Core Records and an Inverse Algorithm

Eckhardt, Sabine; Evangeliou, Nikolaos; Zwaaftink, Christine Groot; Plach, Andreas; McConnell, Joseph; Sigl, Michael; Zdanowicz, Christian; Lim, Seahee; Chellman, Nathan J; Opel, Thomas; Meyer, Hanno; Steffensen, Jørgen Peder; Schwikowski, Margit; Stohl, Andreas

2023

Troll observing network – for useful new data about Antarctica

Pedersen, Christina Alsvik; Njåstad, Birgit; Descamps, Sebastien; Hattermann, Tore; Hudson, Stephen; Flått, Stig; Aas, Wenche; Darelius, Elin Maria K.; Miloch, Wojciech Jacek; Schweitzer, Johannes; Storvold, Rune

2023

Seasonal and latitudinal variability in the atmospheric concentrations of cyclic volatile methyl siloxanes in the Northern Hemisphere

Wania, Frank; Warner, Nicholas Alexander; McLachlan, Michael S; Durham, Jeremy; Lei, Ying Duan; Xu, Shihe

Field data from two latitudinal transects in Europe and Canada were gathered to better characterize the atmospheric fate of three cyclic methylsiloxanes (cVMSs), i.e., octamethyl-cyclotetrasiloxane (D4), decamethylcyclopentasiloxane (D5) and dodecamethylcyclohexasiloxane (D6). During a year-long, seasonally resolved outdoor air sampling campaign, passive samplers with an ultra-clean sorbent were deployed at 15 sampling sites covering latitudes ranging from the source regions (43.7–50.7 °N) to the Arctic (79–82.5 °N). For each site, one of two passive samplers and one of two field blanks were separately extracted and analyzed for the cVMSs at two different laboratories using gas-chromatography-mass spectrometry. Whereas the use of a particular batch of sorbent and the applied cleaning procedure to a large extent controlled the levels of cVMS in field blanks, and therefore also the method detection and quantification limits, minor site-specific differences in field blank contamination were apparent. Excellent agreement between duplicates was obtained, with 95% of the concentrations reported by the two laboratories falling within a factor of 1.6 of each other. Nearly all data show a monotonic relationship between the concentration and distance from the major source regions. Concentrations in source regions were comparatively constant throughout the year, while the concentration gradient towards remote regions became steeper during summer when removal via OH radicals is at its maximum. Concentrations of the different cVMS oligomers were highly correlated within a given transect. Changes in relative abundance of cVMS oligomers along the transect were in agreement with relative atmospheric degradation rates via OH radicals.

2023

Painted Wood Climate Risk Analysis by the HERIe Model of Building Protection and Conservation Heating Scenarios in Norwegian Medieval Stone Churches

Grøntoft, Terje; Stoveland, Lena Porsmo

HERIe was used to model the effect of changes to indoor climate on the risk of humidity-induced mechanical damage (cracking and plastic deformation) to wooden panels painted with stiff gesso in two Norwegian medieval stone churches: Kinn (mean relative humidity (RH, %) = 79%) on the humid west coast, and Ringsaker (mean RH = 49%) in the drier eastern part of the country. The risk involved in moving cultural heritage objects (paint on wood) between the churches and a conservation studio with more “ideal”, stable conditions was also modeled. A hypothetical reduction in RH to ~65% and, proportionally, of the climate fluctuations in Kinn, and an increase in the RH in Ringsaker to a more stable value of ~63% via conservation heating, were found to improve (Kinn) and uphold (Ringsaker) the conformity to relevant standards and significantly reduce the risk of damage, except in the scenario of moving objects from Ringsaker to a conservation studio, when the risk would increase. The use of conservation heating could save ~50% of the heating cost. The estimated risk reductions may be less relevant for objects kept in situ, where cracks in the original paint and gesso have developed historically. They may be more relevant when moving original objects away from their proofed climate into a conservation studio for treatment.

2023

Interim European air quality maps for 2021. PM10, NO2 and ozone spatial estimates based on non-validated UTD data.

Horálek, Jan; Vlasakova, Leona; Schreiberova, Marketa; Schneider, Philipp; Damaskova, Dasa

This report presents European interim air quality maps for 2021, which are based on the non-validated up-to-date (UTD) measurement data and the CAMS Ensemble Forecast modelling results, together with other supplementary data. It contains maps of PM10 and NO2 annual averages and ozone indicator SOMO35.

ETC/HE

2023

European air quality maps for 2020. PM10, PM2.5, Ozone, NO2, NOx and Benzo(a)pyrene spatial estimates and their uncertainties.

Horálek, Jan; Vlasakova, Leona; Schreiberova, Marketa; Markova, Jana; Schneider, Philipp; Kurfürst, Pavel; Tognet, Frédéric; Schovánková, Jana; Vlcek, Ondrej; Damaskova, Dasa

The report provides the annual update of the European air quality concentration maps and population exposure estimates for human health related indicators of pollutants PM10 (annual average, 90.4 percentile of daily means), PM2.5 (annual average), ozone (93.2 percentile of maximum daily 8-hour means, SOMO35, SOMO10), NO2 (annual average) and benzo(a)pyrene (annual average), and vegetation related ozone indicators (AOT40 for vegetation and for forests) for the year 2020. The report contains also Phytotoxic ozone dose (POD) for wheat, potato and tomato maps and NOx annual average map for 2020. The benzo(a)pyrene map is presented for the first time in this regular mapping report. The trends in exposure estimates in the period 2005–2020 are summarized. The analysis for 2020 is based on the interpolation of the annual statistics of the 2020 observational data reported by the EEA member and cooperating countries and other voluntary reporting countries and stored in the Air Quality e-reporting database, complemented, when needed, with measurements from additional sources. The mapping method is the Regression – Interpolation – Merging Mapping (RIMM). It combines monitoring data, chemical transport model results and other supplementary data using linear regression model followed by kriging of its residuals (residual kriging). The paper presents the mapping results and gives an uncertainty analysis of the interpolated maps. It also presents concentration change in 2020 in comparison to the five-year average 2015-2019 using the difference maps.

ETC/HE

2023

Målinger av SO2 i omgivelsene til Elkem Carbon og REC Solar. Januar 2022 – desember 2022.

Hak, Claudia; Stensrød, Anna Maria Røyset; Andresen, Erik

På oppdrag fra Elkem Carbon AS har NILU utført målinger av SO2 i omgivelsene til Elkem Carbon og REC Solar i Vågsbygd (Kristiansand kommune). Elkem Carbon har i sin tillatelse fra Miljødirektoratet krav om å gjennomføre kontinuerlig måling av SO2 i omgivelsesluft. Målingene ble utført med SO2-monitor i boligområdet på Fiskåtangen (Konsul Wilds vei). I tillegg har Elkem Carbon AS valgt å måle med passive SO2-prøvetakere ved 3 steder rundt bedriftene. Rapporten dekker målinger i perioden 1. januar – 31. desember 2022. Norske grenseverdier for luftkvalitet (SO2) ble overholdt ved Konsul Wilds vei for alle midlingsperioder krevet i forurensningsforskriften (årsmiddel, vintermiddel, døgnmiddel og timemiddel). De mest belastede stedene i måleperioden var Konsul Wilds vei nordøst og Fiskåveien rett sør for bedriftene. To døgnmidler var over 125 µg/m3 (grenseverdi, 3 tillatt), 4 døgnmidler var over øvre vurderingsterskel (75 µg/m3) og 11 døgnmidler var over nedre vurderingsterskel (50 µg/m3).

NILU

2023

Deployment and Evaluation of a Network of Open Low-Cost Air Quality Sensor Systems

Schneider, Philipp; Vogt, Matthias; Haugen, Rolf; Hassani, Amirhossein; Castell, Nuria; Dauge, Franck Rene; Bartonova, Alena

Low-cost air quality sensors have the potential to complement the regulatory network of air quality monitoring stations, with respect to increased spatial density of observations, however, their data quality continues to be of concern. Here we report on our experience with a small network of open low-cost sensor systems for air quality, which was deployed in the region of Stavanger, Norway, under Nordic winter conditions. The network consisted of AirSensEUR sensor systems, equipped with sensors for, among others, nitrogen dioxide and fine particulate matter. The systems were co-located at an air quality monitoring station, for a period of approximately six weeks. A subset of the systems was subsequently deployed at various roadside locations for half a year, and finally co-located at the same air quality monitoring station again, for a post-deployment evaluation. For fine particulate matter, the co-location results indicate a good inter-unit consistency, but poor average out-of-the-box performance (R2 = 0.25, RMSE = 9.6 μ
g m−3). While Köhler correction did not significantly improve the accuracy in our study, filtering for high relative humidity conditions improved the results (R2 = 0.63, RMSE = 7.09 μg m−3). For nitrogen dioxide, the inter-unit consistency was found to be excellent, and calibration models were developed which showed good performance during the testing period (on average R2 = 0.98, RMSE = 5.73 μg m−3), however, due to the short training period, the calibration models are likely not able to capture the full annual variability in environmental conditions. A post-deployment co-location showed, respectively, a slight and significant decrease in inter-sensor consistency for fine particulate matter and nitrogen dioxide. We further demonstrate, how observations from even such a small network can be exploited by assimilation in a high-resolution air quality model, thus adding value to both the observations and the model, and ultimately providing a more comprehensive perspective of air quality than is possible from either of the two input datasets alone. Our study provides valuable insights on the operation and performance of an open sensor system for air quality, particularly under challenging Nordic environmental conditions.

2023

CSF to blood clearance differs substantially across individuals and patients with CSF disorders.

Hovd, Markus Herberg; Mariussen, Espen; Uggerud, Hilde Thelle; Lashkarivand, Aslan; Christensen, Hege; Ringstad, Geir; Eide, Per Kristian

2023

Skogens helsetilstand i Norge. Resultater fra skogskadeovervåkingen i 2021

Timmermann, Volkmar; Børja, Isabella; Clarke, Nicholas; Eriksen, Rune; Gohli, Jostein; Hylen, Gro; Jepsen, Jane Uhd; Krokene, Paal; Lange, Holger; Meissner, Helge Rainer; Nagy, Nina Elisabeth; Nordbakken, Jørn-Frode; Solberg, Sverre; Solheim, Halvor; Vindstad, Ole Petter Laksforsmo; Økland, Bjørn; Aas, Wenche

Skogens helsetilstand påvirkes i stor grad av klima og værforhold, enten direkte ved tørke, frost og vind, eller indirekte ved at klimaet påvirker omfanget av soppsykdommer og insektangrep. Klimaendringene og den forventede økningen i klimarelaterte skogskader gir store utfordringer for forvaltningen av framtidas skogressurser. Det samme gjør invaderende skadegjørere, både allerede etablerte arter og nye som kan komme til Norge i nær framtid. I denne rapporten presenteres resultater fra skogskadeovervåkingen i Norge i 2021 og trender over tid for følgende temaer:
(i) Landsrepresentativ skogovervåking;
(ii) Skogøkologiske analyser og målinger av luftkjemi på de intensive overvåkingsflatene;
(iii) Overvåking av bjørkemålere i Troms og Finnmark;
(iv) Barkbilleovervåkingen 2021 og mulig overgang til to generasjoner;
(v) Asiatisk askepraktbille – en dørstokkart?
(vi) Overvåking av askeskuddsyke;
(vii) Andre spesielle skogskader i 2021.

NIBIO

2023

Convention on long-range transboundary air pollution. UN/ECE international operative programme on effects on materials, including historic and cultural monuments. Environmental data report. October 2020 to December 2021

Grøntoft, Terje; Roux, Marta Segura

This report presents the ICP Materials database for the period October 2020 to December 2021. It includes environmental data from the ICP Materials trend exposure programme for 2020 - 2021 and, in addition, data for temperature, relative
humidity, and precipitation amount back to the end of the previous annual exposure programme in October/November 2018. The database consists of meteorological data (T, RH and precipitation amount) and pollution data: Gas concentrations, amounts of ions in precipitation, particle concentrations and amounts of particle deposition.

NILU

2023

Tips og triks for å vinne støvkrigen hjemme

Bohlin-Nizzetto, Pernilla (intervjuobjekt); Lilleås, Vegard (journalist)

2023

Spatial variability and temporal changes of POPs in European background air

Halvorsen, Helene Lunder; Bohlin-Nizzetto, Pernilla; Eckhardt, Sabine; Gusev, Alexey; Möckel, Claudia; Shatalov, Victor; Skogeng, Lovise Pedersen; Breivik, Knut

Concentration data on POPs in air is necessary to assess the effectiveness of international regulations aiming to reduce the emissions of persistent organic pollutants (POPs) into the environment. POPs in European background air are continuously monitored using active- and passive air sampling techniques at a limited number of atmospheric monitoring stations. As a result of the low spatial resolution of such continuous monitoring, there is limited understanding of the main sources controlling the atmospheric burdens of POPs across Europe. The key objectives of this study were to measure the spatial and temporal variability of concentrations of POPs in background air with a high spatial resolution (n = 101) across 33 countries within Europe, and to use observations and models in concert to assess if the measured concentrations are mainly governed by secondary emissions or continuing primary emissions. Hexachlorobenzene (HCB) was not only the POP detected in highest concentrations (median: 67 pg/m3), but also the only POP that had significantly increased over the last decade. HCB was also the only POP that was positively correlated to latitude. For the other targeted POPs, the highest concentrations were observed in the southern part of Europe, and a declining temporal trend was observed. Spatial differences in temporal changes were observed. For example, γ-HCH (hexachlorocyclohexane) had the largest decrease in the south of Europe, while α-HCH had declined the most in central-east Europe. High occurrence of degradation products of the organochlorine pesticides and isomeric ratios indicated past usage. Model predictions of PCB-153 (2,2’,4,4’,5,5’-hexachlorobiphenyl) by the Global EMEP Multi-media Modelling System suggest that secondary emissions are more important than primary emissions in controlling atmospheric burdens, and that the relative importance of primary emissions are more influential in southern Europe compared to northern Europe. Our study highlights the major advantages of combining high spatial resolution observations with mechanistic modelling approaches to provide insights on the relative importance of primary- and secondary emission sources in Europe. Such knowledge is considered vital for policy makers aiming to assess the potential for further emission reduction strategies of legacy POPs.

2023

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